Chapter 7 : DNA Extraction From Plants

DNA extraction is a cornerstone technique in plant molecular biology, allowing scientists to delve into the genetic makeup of various plant species. One popular and efficient method for extracting DNA from plant material is using a silica column. This blog post will guide you through the general steps involved in this process. Materials Required Procedure … Read more

Chapter 6: DNA Extraction From  Animal Tissue

DNA extraction is a fundamental technique in molecular biology, enabling researchers to isolate and study genetic material. When working with animal tissue, a spin column method offers a convenient and efficient way to obtain high-quality DNA. This blog post will outline the general steps involved in DNA extraction from animal tissue using a spin column. … Read more

Chapter 5 : PCR Purification Using Agarose Gel electrophoresis

Polymerase Chain Reaction (PCR) is a cornerstone technique in molecular biology, enabling the amplification of specific DNA sequences. However, PCR reactions often generate unwanted byproducts or non-specific amplification. Gel extraction is a common method used to purify the desired PCR product from these contaminants. What is Gel Extraction? Gel extraction involves separating DNA fragments based … Read more

Chapter 4 : DNA Extraction From Agarose Gel

Agarose gel electrophoresis is a cornerstone technique in molecular biology, used to separate DNA fragments based on size. However, once the DNA is nestled within the gel, how do you extract it for further analysis or manipulation? This blog post will outline a simple and effective method for DNA extraction from agarose gel. Why Extract … Read more

Chapter 3 : DNA Extraction From Blood

Blood DNA Extraction Using Spin Column: A Simple and Efficient Method DNA extraction is a fundamental step in various molecular biology applications, including diagnostics, forensics, and research.  One common source of DNA is blood, and spin column extraction has become a popular method for isolating DNA from blood samples due to its simplicity, speed, and … Read more

Chapter 2 : DNA Extraction From Bacteria

Spin column-based bacterial DNA extraction is a widely used technique in molecular biology due to its speed, efficiency, and ease of use. This method utilizes a silica-based membrane within a spin column to selectively bind DNA in the presence of high salt concentrations.  Bacterial cells are initially lysed to release their contents, and the resulting … Read more

Chapter 1 : Different DNA Extraction Methods

DNA Extraction Methods: Unlocking the Building Blocks of Life DNA extraction is a fundamental process in molecular biology, enabling researchers to isolate and study the genetic material of organisms. Various methods exist for DNA extraction, each tailored to specific sample types and research goals. Common DNA Extraction Methods: Choosing the Right Method: The choice of … Read more